Atlas Taq DNA Polymerase

Atlas RedTaq DNA Polymerase catalyzes 5´ to 3´ synthesis of DNA, but lacks 3´ to 5´exonuclease activity (Fig.1). The enzyme has proven to have high amplification yield, be stable at high temperature. Added inert dye will not have any interference to the reaction. Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred. Samples can be loaded directly onto an agarose gel for electrophoresis with no loading dye addition.

Figure 1. Amplification of 330 bp target DNA using Atlas RedTaq DNA Polymerase.
1- 2 – Atlas RedTaq DNA Polymerase
M – Atlas Star 100 bp DNA Ladder
Concentration: 1 unit/µl
Unit definition: One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70°C.
Source: Recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase.
Quality control: Free of detectable, non-specific nucleases.
Supplied with:
Atlas 10x Taq Buffer: 100 mM Tris-HCl (pH 8.8 at 25°C), 500 mM KCl, 0.8% Nonident P40.
Atlas 10x Taq Buffer with (NH4)2SO4: 750 mM Tris-HCl (pH 8.8 at 25°C),
200 mM (NH4)2SO4, 0.1% Tween 20.
Atlas 25 mM MgCl2
Storage conditions: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol. Store at -20°C. Guaranteed stable for 12 months when properly stored.
Comments: This product is supplied for research use only.