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Covid LAMP Kit ViroReal Kit SARS-CoV2

Covid LAMP Kit ViroReal Kit SARS-CoV2
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Covid LAMP Kit
ViroReal® Kit  SARS-CoV-2 LAMP Kit is now available as ivD test for Covid-19.
Proper native specimens are oropharyngeal swabs (posterior throat swabs) in 2 ml of isotonic saline solution (NaCl 0.9%). There is no need for RNA extraction, because virus inactivation and lysis occur during the isothermal amplification step.
The test is based on one-step RT-LAMP technology (reverse transcription loop-mediated isothermal DNA amplification).
A real-time PCR device can be used for detection of the RT-LAMP reaction. In this case, the generated DNA amplificate can be detected by means of an intercalating fluorescent dye by amplification and melting curves. This test is compatible with real-time PCR instruments which detect fluorescence in FAM channel.
Alternatively, a conventional block PCR device can be used.
After amplification, a visual check of the color change in the reaction tubes must be performed (detection by change of color from red to yellow, based on the pH indicator dye used in the LAMP reaction). The drop in pH value is due to the large amount of generated DNA.
The amplification takes 30 min at 63°C. The melting curve analysis takes between 5-13 minutes.
Samples with an RNA concentration of approx. 20,000 copies per ml (corresponds to approx. 100 copies per RT-LAMP reaction and a real-time PCR Cq value of approx. 31) can be reliably detected. Patients with a lower viral load are most likely not infectious (Wölfel et al., 2020 and recommendation of Robert Koch-Institute).


 
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