NucleoBond 96 Xtra EF, 96‑well kit for endotoxin-free plasmid DNA

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2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA

Application: Endotoxin-free plasmid DNA isolation
Target: Plasmid DNA
CE-zertifiziert: No, research use only
Technology: Anion exchange chromatography
Brand: NucleoBond
Format: 96-well plate
Verpackungseinheit: 96 Prep(s), 384 Prep(s)
Handling: Gravity flow , vacuum for filtration and precipitation
Lysate clarification: NucleoBond Filter Plate
Automated use. No
Sample material: Bacteria, E. coli
Sample amount: 1−5 mL
Vector size: < 25 kbp; < 300 kbp (without the NucleoBond Finalizer Plate)
Typical yield: 2−4 µg (1.5 mL in 96-well plates), 10−50 µg (5 mL in glass tubes)
Theoretical binding capacity: 50 µg
Typical purity A260/A280: 1.8−1.95
Endotoxin level: < 0.1 EU/µg DNA
Isopropanol precipitation: Centrifugation, NucleoBond Finalizer Plate
Elutionsvolumen: 100−200 µL
Preparation time: 120 min/plate
Typical downstream application: Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Lagertemperatur: 18−25 °C
Haltbarkeit (ab Produktion): 39 Monate
Gefahrstoffe: Ja