NucleoBond PC 2000 EF, Mega kit for endotoxin-free plasmid DNA

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1st generation anion exchange technology for endotoxin-free plasmid DNA

Application: High and low copy plasmid DNA purification
Target: Plasmid DNA
CE-zertifiziert: No, research use only
Technology: Anion exchange chromatography
Brand: NucleoBond
Format: Mega prep
Verpackungseinheit: 5 Prep(s)
Handling: Gravity flow
Lysate clarification: Bottle top filter
Automated use: No
Sample material: Bacteria, E. coli
Sample amount: 150−500 mL
Vector size: < 300 kbp
Typical yield: 500−2000 µg
Theoretical binding capacity: 2000 µg
Typical purity A260/A280: 1.8−1.95
Endotoxin level: < 0.1 EU/µg DNA
Isopropanol precipitation: Centrifugation, NucleoBond Finalizer Large, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Preparation time: 150 min/2 preps
Typical downstream application: Automated or manual sequencing, Cloning, hybridization, In-vitro transcription, PCR, Transfection
Lagertemperatur: 18−25 °C
Haltbarkeit (ab Produktion): 27 Monate
Lieferumfang: NucleoBond AX 2000 Columns, NucleoBond Bottle Top Filters Type 1, buffers, RNase A
Gefahrstoffe: Ja