NucleoBond PC preparative scale purification of endotoxin-free plasmid DNA

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Preparative scale isolation of endotoxin-free plasmid DNA

Application: High and low copy plasmid DNA purification
Target: Plasmid DNA
CE-zertifiziert: No, research use only
Technology: Anion exchange chromatography
Brand: NucleoBond
Format: Preparative scale
Verpackungseinheit: 1 Prep(s)
Handling: HPLC, FPLC
Lysate clarification: Folded filter, sieving fabric
Automated use: No
Sample material: Bacteria, E. coli
Sample amount: 5−20 L
Vector size: < 300 kbp
Typical yield: 80−100 mg
Theoretical binding capacity: 100 mg
Typical purity A260/A280: 1.8−1.95
Endotoxin level: < 0.1 EU/µg DNA
Isopropanol precipitation: Centrifugation
Preparation time: 20 h/prep
Typical downstream application: Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Lagertemperatur: 18−25 °C
Haltbarkeit (ab Produktion): 39 Monate
Lieferumfang: NucleoBond AX Prep 100 Column, NucleoBond AX 100 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Gefahrstoffe: Ja