NoFluor Diaminofluorescein-FM diacetate (DAF-FM DA) Cell-permeable fluorescence

NoFluor Diaminofluorescein-FM diacetate (DAF-FM DA) Cell-permeable fluorescence
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High fluorescence intensity even at low pH (around pH 6) acidic  conditions.
Permeable through the cell membrane and real time observation of NO inside tissues or cultured cells is available.
Long time localization inside the cell.
Easily observed by a fluorescence microscopy.
Low damage to the cells because the reagent is excited by the visible light.
High sensitivity and selectivity.
Principle of measurement
After the permeation through the cell membrane, DAF-FM DA is hydrolyzed by intracellular esterase to DAF-FM which is unpermeable through the cell membrane. Amino groups of DAF-FM capture NO, and the DAF-FM makes fluorescence of green light with wave length of 515 nm when it’s excited by the light with wavelength of 500 nm.
DAF-FM DA 1mg (in DMSO 0.4 mL)
C25H18F2N2O7             Mw:496.42
Preparation of  the reagent
Density of the provided sample is 5 mmol/L in DMSO. Dilute 500 times with neutral buffer before use.