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NoFluor Diaminofluorescein-4M acetoxymethyl ester (DAR-4M AM) probe

NoFluor Diaminofluorescein-4M acetoxymethyl ester (DAR-4M AM) probe
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Features
Permeable through the cell membrane and real time observation (Cell permeabvle Orange Fluorescence) of NO inside tissues or cultured cells is available.
Low autofluorescence from the living sample because the reagent is excited by the light with long wave length.
High intensity of fluorescence at the wide range of pH (from pH 4 to 12).
Principle of the measurement
Amino groups of Diaminorhodamine react with NO, and it makes fluorescence of green light with wave length of 575 nm when it’s excited by the light with wavelength of 560 nm.
Contents
DAR-4M AM1mg (in DMSO 0.32 mL)
C28H31IN4O5            Mw:630.47
Preparation of the reagent
Density of the provided sample is 5 mmol/L in DMSO. Dilute 500 times with neutral buffer before use.
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